Background: Urinary bladder is an organ which incessantly exposed to carcinogenic substances and may cause chronic inflammation may subsequently lead to tumorigenesis. The functional regulatory polymorphisms of TNF-alpha gene modulate its control of inflammation and other major functions that may eventually lead to tumorigenesis. Objective: To analyze the link between three genetic polymorphisms of TNF-alpha gene including -863C > A, -308 G > A and - 238 G > A and the risk of developing UBC in Indian population. Methods: Direct DNA sequencing method was performed for the assessment of genotyping of TNF-alpha gene polymorphisms in the blood samples of histopathologically confirmed 232 cases of UBC compared to cancer free 250 controls. We have conducted meta-analysis investigating the association between TNF-alpha -308 G > A polymorphism and the risk of UBC by using available data from six studies (including the present data), comprising 1397 cases and 1722 controls. Results: Dominant model of -863C > A showed 2.33 fold higher risk of UBC in cases as compared to controls (p = 0.00003; OR = 2.33, 95%CI 1.56-3.47) while contrary, recessive model showed a significant protective effect (p = 0.03; OR = 0.45, 95%CI 0.23-0.91). Other polymorphism -238 G > A and - 308 G > A did not reveal significant association with UBC risk. Meta-analysis study did not show any significant association with the risk of UBC development (OR = 1.01, 95%CI 0.84-1.23; p = 0.88) with the fixed effect model (OR = 1.01, 95%CI 0.84-1.23; p = 0.88) random effect model. Conclusion: Overall, the results suggest that TNF-alpha -863C > A polymorphism has been found to significantly influence the risk of UBC. Further researches are needed to understand its potential role on UBC risk.